e2 protein-based elisa Search Results


human granzyme b  (R&D Systems)
93
R&D Systems human granzyme b
Increased Vδ1 T cell-cytotoxicity against Colo357 cells by bsAb [HER2xCD3] is further enhanced by COX inhibitors. Ten thousand control- or TRAIL-R (TR) 4 KD Colo357 cells were incubated for 24 h in 96-well E-plates in medium. After 23 h, a final concentration of 1 μg/mL bsAb [HER2xCD3] or 50 μM Indomethacin alone or in combination or alternatively in combination with 30 μM DuP697 or 2.5 mM valeryl salicylate as indicated were added. After 24 h, Vδ1 T cell lines of six different donors with an E/T ratio of 25:1 and a final concentration of 12.5 U/mL IL-2 were cocultured. (A) The release of <t>granzyme</t> <t>B</t> or (B) PGE2 were measured after 24 h by ELISA and (C) Vδ1 T cell-mediated cytotoxicity against Colo357 cells by RTCA shown as decrease in Cell Index (CI) fold change calculated with the formula mentioned in . Black bars represent mean of experiments. Significances are shown as P -value; ** = P < 0.01.
Human Granzyme B, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e2 protein-based elisa  (IDEXX)
90
IDEXX e2 protein-based elisa
Increased Vδ1 T cell-cytotoxicity against Colo357 cells by bsAb [HER2xCD3] is further enhanced by COX inhibitors. Ten thousand control- or TRAIL-R (TR) 4 KD Colo357 cells were incubated for 24 h in 96-well E-plates in medium. After 23 h, a final concentration of 1 μg/mL bsAb [HER2xCD3] or 50 μM Indomethacin alone or in combination or alternatively in combination with 30 μM DuP697 or 2.5 mM valeryl salicylate as indicated were added. After 24 h, Vδ1 T cell lines of six different donors with an E/T ratio of 25:1 and a final concentration of 12.5 U/mL IL-2 were cocultured. (A) The release of <t>granzyme</t> <t>B</t> or (B) PGE2 were measured after 24 h by ELISA and (C) Vδ1 T cell-mediated cytotoxicity against Colo357 cells by RTCA shown as decrease in Cell Index (CI) fold change calculated with the formula mentioned in . Black bars represent mean of experiments. Significances are shown as P -value; ** = P < 0.01.
E2 Protein Based Elisa, supplied by IDEXX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e2 protein-based elisa - by Bioz Stars, 2026-03
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blocking elisa kit  (IDEXX)
90
IDEXX blocking elisa kit
Increased Vδ1 T cell-cytotoxicity against Colo357 cells by bsAb [HER2xCD3] is further enhanced by COX inhibitors. Ten thousand control- or TRAIL-R (TR) 4 KD Colo357 cells were incubated for 24 h in 96-well E-plates in medium. After 23 h, a final concentration of 1 μg/mL bsAb [HER2xCD3] or 50 μM Indomethacin alone or in combination or alternatively in combination with 30 μM DuP697 or 2.5 mM valeryl salicylate as indicated were added. After 24 h, Vδ1 T cell lines of six different donors with an E/T ratio of 25:1 and a final concentration of 12.5 U/mL IL-2 were cocultured. (A) The release of <t>granzyme</t> <t>B</t> or (B) PGE2 were measured after 24 h by ELISA and (C) Vδ1 T cell-mediated cytotoxicity against Colo357 cells by RTCA shown as decrease in Cell Index (CI) fold change calculated with the formula mentioned in . Black bars represent mean of experiments. Significances are shown as P -value; ** = P < 0.01.
Blocking Elisa Kit, supplied by IDEXX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/blocking elisa kit/product/IDEXX
Average 90 stars, based on 1 article reviews
blocking elisa kit - by Bioz Stars, 2026-03
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serum  (Valiant Co Ltd)
99
Valiant Co Ltd serum
Increased Vδ1 T cell-cytotoxicity against Colo357 cells by bsAb [HER2xCD3] is further enhanced by COX inhibitors. Ten thousand control- or TRAIL-R (TR) 4 KD Colo357 cells were incubated for 24 h in 96-well E-plates in medium. After 23 h, a final concentration of 1 μg/mL bsAb [HER2xCD3] or 50 μM Indomethacin alone or in combination or alternatively in combination with 30 μM DuP697 or 2.5 mM valeryl salicylate as indicated were added. After 24 h, Vδ1 T cell lines of six different donors with an E/T ratio of 25:1 and a final concentration of 12.5 U/mL IL-2 were cocultured. (A) The release of <t>granzyme</t> <t>B</t> or (B) PGE2 were measured after 24 h by ELISA and (C) Vδ1 T cell-mediated cytotoxicity against Colo357 cells by RTCA shown as decrease in Cell Index (CI) fold change calculated with the formula mentioned in . Black bars represent mean of experiments. Significances are shown as P -value; ** = P < 0.01.
Serum, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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serum - by Bioz Stars, 2026-03
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hev igm elisa 3 0  (Valiant Co Ltd)
93
Valiant Co Ltd hev igm elisa 3 0
Increased Vδ1 T cell-cytotoxicity against Colo357 cells by bsAb [HER2xCD3] is further enhanced by COX inhibitors. Ten thousand control- or TRAIL-R (TR) 4 KD Colo357 cells were incubated for 24 h in 96-well E-plates in medium. After 23 h, a final concentration of 1 μg/mL bsAb [HER2xCD3] or 50 μM Indomethacin alone or in combination or alternatively in combination with 30 μM DuP697 or 2.5 mM valeryl salicylate as indicated were added. After 24 h, Vδ1 T cell lines of six different donors with an E/T ratio of 25:1 and a final concentration of 12.5 U/mL IL-2 were cocultured. (A) The release of <t>granzyme</t> <t>B</t> or (B) PGE2 were measured after 24 h by ELISA and (C) Vδ1 T cell-mediated cytotoxicity against Colo357 cells by RTCA shown as decrease in Cell Index (CI) fold change calculated with the formula mentioned in . Black bars represent mean of experiments. Significances are shown as P -value; ** = P < 0.01.
Hev Igm Elisa 3 0, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hev igm elisa 3 0/product/Valiant Co Ltd
Average 93 stars, based on 1 article reviews
hev igm elisa 3 0 - by Bioz Stars, 2026-03
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elisa kits for csfv antibody detection  (IDEXX)
90
IDEXX elisa kits for csfv antibody detection
Expression and identification of the <t>CSFV</t> <t>E2</t> protein. (A) Results of 12% SDS-PAGE. Lane M, prestained protein markers; lanes 1 to 6, transfected cells; lane 7, untransfected normal cells. (B) Results of WB. Lane M, prestained protein markers; lanes 1 to 7 are the same as described for panel A. (C) Validation of the immunogenicity of the CSFV E2 protein.
Elisa Kits For Csfv Antibody Detection, supplied by IDEXX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kits for csfv antibody detection - by Bioz Stars, 2026-03
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lipofectaminetm 2000  (Fisher Scientific)
90
Fisher Scientific lipofectaminetm 2000
Expression and identification of the <t>CSFV</t> <t>E2</t> protein. (A) Results of 12% SDS-PAGE. Lane M, prestained protein markers; lanes 1 to 6, transfected cells; lane 7, untransfected normal cells. (B) Results of WB. Lane M, prestained protein markers; lanes 1 to 7 are the same as described for panel A. (C) Validation of the immunogenicity of the CSFV E2 protein.
Lipofectaminetm 2000, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse pge2 elisa  (Cayman Chemical)
90
Cayman Chemical mouse pge2 elisa
Expression and identification of the <t>CSFV</t> <t>E2</t> protein. (A) Results of 12% SDS-PAGE. Lane M, prestained protein markers; lanes 1 to 6, transfected cells; lane 7, untransfected normal cells. (B) Results of WB. Lane M, prestained protein markers; lanes 1 to 7 are the same as described for panel A. (C) Validation of the immunogenicity of the CSFV E2 protein.
Mouse Pge2 Elisa, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
mouse pge2 elisa - by Bioz Stars, 2026-03
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bca protein assay kit  (Fisher Scientific)
90
Fisher Scientific bca protein assay kit
Expression and identification of the <t>CSFV</t> <t>E2</t> protein. (A) Results of 12% SDS-PAGE. Lane M, prestained protein markers; lanes 1 to 6, transfected cells; lane 7, untransfected normal cells. (B) Results of WB. Lane M, prestained protein markers; lanes 1 to 7 are the same as described for panel A. (C) Validation of the immunogenicity of the CSFV E2 protein.
Bca Protein Assay Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
bca protein assay kit - by Bioz Stars, 2026-03
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rneasy mini kit  (Qiagen)
90
Qiagen rneasy mini kit
Expression and identification of the <t>CSFV</t> <t>E2</t> protein. (A) Results of 12% SDS-PAGE. Lane M, prestained protein markers; lanes 1 to 6, transfected cells; lane 7, untransfected normal cells. (B) Results of WB. Lane M, prestained protein markers; lanes 1 to 7 are the same as described for panel A. (C) Validation of the immunogenicity of the CSFV E2 protein.
Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
rneasy mini kit - by Bioz Stars, 2026-03
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realtime-glo annexin v apoptosis assay  (Promega)
90
Promega realtime-glo annexin v apoptosis assay
Expression and identification of the <t>CSFV</t> <t>E2</t> protein. (A) Results of 12% SDS-PAGE. Lane M, prestained protein markers; lanes 1 to 6, transfected cells; lane 7, untransfected normal cells. (B) Results of WB. Lane M, prestained protein markers; lanes 1 to 7 are the same as described for panel A. (C) Validation of the immunogenicity of the CSFV E2 protein.
Realtime Glo Annexin V Apoptosis Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matrigel gfr  (Corning Life Sciences)
90
Corning Life Sciences matrigel gfr
Expression and identification of the <t>CSFV</t> <t>E2</t> protein. (A) Results of 12% SDS-PAGE. Lane M, prestained protein markers; lanes 1 to 6, transfected cells; lane 7, untransfected normal cells. (B) Results of WB. Lane M, prestained protein markers; lanes 1 to 7 are the same as described for panel A. (C) Validation of the immunogenicity of the CSFV E2 protein.
Matrigel Gfr, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Increased Vδ1 T cell-cytotoxicity against Colo357 cells by bsAb [HER2xCD3] is further enhanced by COX inhibitors. Ten thousand control- or TRAIL-R (TR) 4 KD Colo357 cells were incubated for 24 h in 96-well E-plates in medium. After 23 h, a final concentration of 1 μg/mL bsAb [HER2xCD3] or 50 μM Indomethacin alone or in combination or alternatively in combination with 30 μM DuP697 or 2.5 mM valeryl salicylate as indicated were added. After 24 h, Vδ1 T cell lines of six different donors with an E/T ratio of 25:1 and a final concentration of 12.5 U/mL IL-2 were cocultured. (A) The release of granzyme B or (B) PGE2 were measured after 24 h by ELISA and (C) Vδ1 T cell-mediated cytotoxicity against Colo357 cells by RTCA shown as decrease in Cell Index (CI) fold change calculated with the formula mentioned in . Black bars represent mean of experiments. Significances are shown as P -value; ** = P < 0.01.

Journal: Frontiers in Immunology

Article Title: TRAIL-Receptor 4 Modulates γδ T Cell-Cytotoxicity Toward Cancer Cells

doi: 10.3389/fimmu.2019.02044

Figure Lengend Snippet: Increased Vδ1 T cell-cytotoxicity against Colo357 cells by bsAb [HER2xCD3] is further enhanced by COX inhibitors. Ten thousand control- or TRAIL-R (TR) 4 KD Colo357 cells were incubated for 24 h in 96-well E-plates in medium. After 23 h, a final concentration of 1 μg/mL bsAb [HER2xCD3] or 50 μM Indomethacin alone or in combination or alternatively in combination with 30 μM DuP697 or 2.5 mM valeryl salicylate as indicated were added. After 24 h, Vδ1 T cell lines of six different donors with an E/T ratio of 25:1 and a final concentration of 12.5 U/mL IL-2 were cocultured. (A) The release of granzyme B or (B) PGE2 were measured after 24 h by ELISA and (C) Vδ1 T cell-mediated cytotoxicity against Colo357 cells by RTCA shown as decrease in Cell Index (CI) fold change calculated with the formula mentioned in . Black bars represent mean of experiments. Significances are shown as P -value; ** = P < 0.01.

Article Snippet: PGE2 was measured by Prostaglandin E2 Parameter Assay Kit (#SKGE004B) and human granzyme B by a sensitive sandwich ELISA (both from R&D System) in duplicates following the procedures outlined by the manufacturer.

Techniques: Incubation, Concentration Assay, Enzyme-linked Immunosorbent Assay

Expression and identification of the CSFV E2 protein. (A) Results of 12% SDS-PAGE. Lane M, prestained protein markers; lanes 1 to 6, transfected cells; lane 7, untransfected normal cells. (B) Results of WB. Lane M, prestained protein markers; lanes 1 to 7 are the same as described for panel A. (C) Validation of the immunogenicity of the CSFV E2 protein.

Journal: Microbiology Spectrum

Article Title: A Novel Blocking Enzyme-Linked Immunosorbent Assay Based on a Biotinylated Nanobody for the Rapid and Sensitive Clinical Detection of Classical Swine Fever Virus Antibodies

doi: 10.1128/spectrum.02996-22

Figure Lengend Snippet: Expression and identification of the CSFV E2 protein. (A) Results of 12% SDS-PAGE. Lane M, prestained protein markers; lanes 1 to 6, transfected cells; lane 7, untransfected normal cells. (B) Results of WB. Lane M, prestained protein markers; lanes 1 to 7 are the same as described for panel A. (C) Validation of the immunogenicity of the CSFV E2 protein.

Article Snippet: Several companies have developed ELISA kits for CSFV antibody detection based on the CSFV E2 protein, and examples of these companies include Biocheck (catalog no. SK106 CSFV E2), Cusabio Technology LLC (catalog no. CSB-E12765p), IDEXX Laboratories (catalog no. 99-43220), ID VET (catalog no. CSFE2C-5P), and Thermo Fisher Scientific (catalog no. 7610046) ( ).

Techniques: Expressing, SDS Page, Transfection, Biomarker Discovery, Immunopeptidomics

Screening Nbs against CSFV E2 protein. (A) Analysis of the binding ability of recombinant Nbs against E2 by iELISA. (B) Functional region division and amino acid sequence analysis of 10 Nbs.

Journal: Microbiology Spectrum

Article Title: A Novel Blocking Enzyme-Linked Immunosorbent Assay Based on a Biotinylated Nanobody for the Rapid and Sensitive Clinical Detection of Classical Swine Fever Virus Antibodies

doi: 10.1128/spectrum.02996-22

Figure Lengend Snippet: Screening Nbs against CSFV E2 protein. (A) Analysis of the binding ability of recombinant Nbs against E2 by iELISA. (B) Functional region division and amino acid sequence analysis of 10 Nbs.

Article Snippet: Several companies have developed ELISA kits for CSFV antibody detection based on the CSFV E2 protein, and examples of these companies include Biocheck (catalog no. SK106 CSFV E2), Cusabio Technology LLC (catalog no. CSB-E12765p), IDEXX Laboratories (catalog no. 99-43220), ID VET (catalog no. CSFE2C-5P), and Thermo Fisher Scientific (catalog no. 7610046) ( ).

Techniques: Binding Assay, Recombinant, Functional Assay, Sequencing

Optimized reaction conditions for the developed bELISA. (A) Nb1-biotin titer. (B) Screening of optimal blocking solutions. (C) Screening of optimal dilutions of CSFV E2 protein and Nb1-biotin. (D) Screening of optimal serum dilution. (E) Screening of optimal serum incubation, Nb1-biotin incubation, and HRP-streptavidin incubation times. (F) Distribution of the PI values obtained from the detection of CSFV antibody-negative porcine sera with the developed bELISA method.

Journal: Microbiology Spectrum

Article Title: A Novel Blocking Enzyme-Linked Immunosorbent Assay Based on a Biotinylated Nanobody for the Rapid and Sensitive Clinical Detection of Classical Swine Fever Virus Antibodies

doi: 10.1128/spectrum.02996-22

Figure Lengend Snippet: Optimized reaction conditions for the developed bELISA. (A) Nb1-biotin titer. (B) Screening of optimal blocking solutions. (C) Screening of optimal dilutions of CSFV E2 protein and Nb1-biotin. (D) Screening of optimal serum dilution. (E) Screening of optimal serum incubation, Nb1-biotin incubation, and HRP-streptavidin incubation times. (F) Distribution of the PI values obtained from the detection of CSFV antibody-negative porcine sera with the developed bELISA method.

Article Snippet: Several companies have developed ELISA kits for CSFV antibody detection based on the CSFV E2 protein, and examples of these companies include Biocheck (catalog no. SK106 CSFV E2), Cusabio Technology LLC (catalog no. CSB-E12765p), IDEXX Laboratories (catalog no. 99-43220), ID VET (catalog no. CSFE2C-5P), and Thermo Fisher Scientific (catalog no. 7610046) ( ).

Techniques: Blocking Assay, Incubation